The Three Eagle Trail is a non-motorized biking and walking path connecting the communities of Three Lakes and Eagle River. The basic route is described below. In the winter ski season part of the trail is groomed for cross country skiing as described farther below.

From the Historic Rail Depot in Eagle River the Trail heads south past the Dairy Queen and follows a ten foot wide, crushed limestone path along the former Chicago & Northwestern Railroad grade for about 1.5 miles. The Trail then leads through a mix of open land and forest to an impressive, 660 foot long boardwalk and bridge. Beyond the boardwalk the trail continues on to a wooded upland, terminating at a rustic rest area almost 3 miles south of the DQ near Section 9 Road. This trailhead, marking a break in the non-motorized part of the Trail is about 1.5 miles north of the Sundstein Road Trailhead where the non-motorized pathway continues for another 8.4 miles to Three Lakes. This part of the Trail winds through a beautiful landscape of woodlands and wetlands. Features along the way include a bridge and two scenic boardwalks. The terrain is level to gently rolling. The area is quiet and remote. Be prepared with water, a first aid kit, insect protection and bike repair tools. You may also want to bring a camera. The southernmost stretch of the Trail follows the former Chicago & Northwestern Railroad grade for 3.5 miles to Don Burnside Park in Three Lakes.

In the winter season, the middle 5 miles of the Trail are groomed for cross country skiing with a skate lane and classic tracks. The ski trail can be accessed from a small parking area on the west side of Highway 32/45, just 3 miles north of the Three Lakes business district, or from the Sundstein Road parking area, about four miles south of Eagle River. Also in winter, there are marked snowshoe trails at both ends of the Cross Country Ski Trail and near the Section 9 Road Trailhead Rest Area, about 3 miles south of Eagle River on Sundstein Road and then east on Section 9 Road about ¼ mile.